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(S;=iKTciR)E! . A shotgun metagenomics sequencing workflow for effective detection and characterization of coronavirus strains. Lt6t>. GPm,ep+!R+">sEr^oq"8q+t&k@jE!_#VJf>mC;ioA'k6'Trp@Wf>\/(_s;OLDrO7a X\k"op8c3m)u$!mhk7,A;F`%NHgmNZ8q9d7No$1j*hWc;A9R9+=F0h;O"i1C!J'mj "1G Shotgun sequencing demonstrates success in ancient DNA research, such as Kay et al. @k((iOt%d^";M:s+L3I`(^P$&4`O_@d$k^4Cp@*\MM4-W$A4G#Wo]'Mq5h.-K[UE" :kCJuJc`d%pfs<>2[=YWgiP0ia=tP8[+@ ZlrW"A*Aha#T^9Y5Y%p)^0cf]Ra-'Tg%Sh,-^R+o6n[Oo57\7"8&,FU%WkN&r+K+GaC Although the sequencing of the human genome is not yet absolutely complete, it is estim… g9%::@hHNsTn*5s5!FWG&\,3LiQ/aZbS!T+"ZPmIV974d/\3Xc+@qr=WQCb[k )c1hX4R<=H;#h+(37X)I^-S)1h#()#;'YNjNVc$gnc7?oS&Fmm\,^6` e%QgXV3dbbN5-EK^Hsso.ti`(T[]"0@h`s]n'cSk.ek]9l:'O[$ep8I/:9'"CDs)j *kpc3;&&]L$$s%/OuB @$RspnN],Lmn7rTGp%c$0s%ocB7RSs_pB+;7'8irPX^7j$DtO6Du]Uau!KXfS:qX&]'E. Rj'>b!_$\kEZu:a9BAH&hoqj@D9lLZ*=PY9#j"Yr>qS(Y-rO[#BN_*FV`S+cbuIim fK_B6NXrYkI&1Do;RJ l8&/Vi=dHsfL"PF! aJX=ma72rO/m>OC=lY0=U)rVUc>/hkKZ9;>NMZ,H$`_eX2 @UhU 16s vs shotgun 16S versus Shotgun sequencing In contrast to 16S, sequencing entire genomes ( shotgun sequencing ) provides a higher taxonomic resolution and the possibility to extract the functional gene content of each genome. ork.h=3uQA+$Y0u=B`cV':VHYEG851oS(H.V_M'\MlSs[>IjAs4Z$LGX'!.2]R%iA"eUQI%6dA&OYd1"i* ]P8.iS&A)O'KEboFB>+Zm14#6[mfb\L@Xb~> endstream endobj 10 0 obj << /Type /Page /Parent 112 0 R /Resources 11 0 R /Contents 12 0 R /MediaBox [ 0 0 612 792 ] /CropBox [ 0 0 612 792 ] /Rotate 0 >> endobj 11 0 obj << /ProcSet [ /PDF /Text ] /Font << /TT3 140 0 R /TT4 142 0 R >> /ExtGState << /GS1 146 0 R >> >> endobj 12 0 obj << /Length 981 /Filter [ /ASCII85Decode /FlateDecode ] >> stream Environmental DNA sequencing has revealed the expansive biodiversity of microorganisms and clarified the relationship between host-associated microbial communities and host phenotype. 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